FIGURE 4.

Macroglial activation in the diabetic retina at 8 weeks after STZ (streptozotocin) injection. (a) Glial fibrillary acidic protein (GFAP) immunoreactivity spanned the entire retina vertically to the outer plexiform layer in the diabetes group, and this effect was attenuated by the combined treatment. (b) Increased GFAP immunostaining was reduced by the brinzolamide or the combined treatment (n = 10 per group). (c) In western blot analysis, elevated GFAP expression in the diabetes group decreased in the combined treatment group (n = 10 per group). *P < .05, significantly different as indicated; Kruskal–Wallis and post hoc Dunn's test (one‐tailed)