FIGURE 5.

Effect of PDE inhibitors on the inotropic potency of isoprenaline in electrically stimulated (1.5–2.0 Hz) hiPSC‐CM EHTs, expressed as LogEC₅₀ for isoprenaline of data shown in Figure 4. Scatter plot of raw data with mixed model‐estimated marginal means and 95% confidence intervals; P < .05, significantly different from vehicle control (VC); pairwise comparisons (sequentially step‐down rejective Bonferroni‐adjusted). VC: DMSO 0.1%, n = 45; 6); Mil: milrinone (10 μM, n = 17; 3); Cil: cilostamide (300 nM, n = 22; 3); Rol: rolipram (10 μM, n = 10; 2); Tad: tadalafil (10 nM, n = 10; 2); IBMX (10 μM, n = 15; 3), n; n = number of EHTs; number of differentiation batches, the number of EHTs were used for statistics. F test results (F values with dfmean;dferror in brackets) with P value for the effect of condition: P _{condition (VC, Mil, Cil, Rol, Tad, IBMX)} < .001 (F[5;113] = 9.744). F test results were calculated from the adjusted data set. See Section 2 for further information on data analytical procedures