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. 2020 Mar 23;177(13):2959–2973. doi: 10.1111/bph.15023

FIGURE 2.

FIGURE 2

JQ1 suppresses p300‐mediated acetylation of RORγt. HEK293T cells were transfected with Flag‐p300 and Myc‐RORγt (a) or Myc‐RORγt plasmid alone (b). Thirty‐nine hours later, cells were treated with or without JQ1 as indicated for 9 hr. Cell lysates were immunoprecipitated with anti‐Flag antibodies and submitted for immunoblotting with the indicated antibodies. (a,b) Representative images from five independent experiments are shown in the left panels. In the right panels, the corresponding graphs show the average of protein relative level quantified by densitometry. (c) Dose‐dependent effect of JQ1 on the viability of HEK293T cells. HEK293T cells were treated with JQ1 at indicated concentration for 3 hr. Cell viability was determined using the CCK8 assay kit. Results are presented as mean ± SEM from five independent experiments: NS, no significant, n = 5, * P < .05, compared to DMSO treatment (one‐way ANOVA)