FIGURE 4.

Identification of a new peroxidase gene as a direct target of BSR‐D1. (a) Binding of BSR‐D1 to the promoters of seven reduction‐oxidation reaction‐associated genes in a yeast one‐hybrid assay. Each promoter was fused to the pHIS2 reporter and BSR‐D1 was fused to GAL4 AD. Yeast cells were transformed with the reporter and effector constructs with or without Bsr‐d1. (b) In vitro pull‐down of target DNA by BSR‐D1. GST‐BSR‐D1 or GST alone were incubated with total rice DNA and subjected to quantitative PCR for the Perox3 gene. The fold enrichment was normalized against the Ub promoter. Each bar represents the mean and SD of three repeats. *p < .01. (c) RNA expression levels of the peroxidase gene (LOC_Os01g73170, named as Perox3) in Bsr‐d1 knockout (Bsr‐d1KO) plants. The expression levels are normalized to the Ubq5 reference gene. RNA was prepared from leaf samples at the three‐leaf stage. Error bars represent the SD from three replicates