FIGURE 1.

Infection of TSWV‐LE and TSWV‐YN in Arabidopsis thaliana Col‐0 plants at different growth stages. (a) Arabidopsis Col‐0 plants at different growth stages were rub‐inoculated with a TSWV‐LE‐ or TSWV‐YN‐infected crude leaf extract. A representative plant was selected from each treatment and photographed at 30 days post‐inoculation (dpi). Plants inoculated with phosphate‐buffered saline (PBS) were used as negative (Mock) controls. The numbers in yellow are the total number of inoculated plants versus noninfected (3‐, 4‐, 5‐, and 6‐week‐old) or infected (7‐ and 8‐week‐old) plants observed from various treatments. (b) and (d) Newly emerged leaves were harvested from the representative plants (a) and analysed for TSWV‐LE or TSWV‐YN infection through reverse transcription (RT)‐PCR. The 3‐, 4‐, 5‐, and 6‐week‐old inoculated plants were analysed at 30 dpi, and the 7‐ and 8‐week‐old inoculated plants were analysed at 15 dpi. Total RNA isolated from Arabidopsis Col‐0 plants inoculated with PBS at the 8‐week‐old stage was used as a negative control. (c) and (e) RT‐PCR results showing the numbers of TSWV‐LE‐ or TSWV‐YN‐infected (red) or uninfected (blue) Arabidopsis plants inoculated at different developmental stages. A total of 24 plants were used for each treatment