Figure 10.

Berberine (BBR)-NLCs blocked LPS-induced NF-κB nuclear translocation.

Notes: (A) Untreated RAW264.7 cells. (B) RAW264.7 cells treated with LPS (100 ng/mL) for 6 h. (C) RAW264.7 cells pretreated with BBR (5 μg/mL) for 2 h, then treated with LPS (100 ng/mL) for 6 h. (D) RAW264.7 cells pretreated with BBR-NLCs (5 μg/mL) for 2 h, then treated with LPS (100 ng/mL) for 6 h. The translocation of NF-κB was determined using immunofluorescence. For all experiments, n = 3, and the scale bar = 20 µm.