Figure 2.

Nucleosome remodeling and histone deacetylation (NuRD) complex and cardiac gene regulation. (A) The NuRD complex interacts with FOG2, a direct modulator of GATA4 transcriptional activity. One target of GATA4–FOG2–NuRD repression was Cdkn1a. (B) TBX5 directly interacts with NuRD complex subunits CHD4, HDAC2, and MTA1 to repress gene expression programs not normally expressed during heart development. TBX5 mutations in its NuRD interaction domain (NID) abolished direct interaction with the NuRD complex, leading to expression of normally repressed, ectopic gene programs. (C) Physical interaction between TBX20 and the NuRD complex is mediated through Gro/TLE corepressors. Specifically, the TBX20 eh1 domain is essential for proper transcriptional regulation by the Gro/TLE–NuRD complexes.