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. 2020 Feb 28;10(5):861–877. doi: 10.1016/j.apsb.2020.02.009

Figure 2.

Figure 2

Pretreatment with etanercept protected mice from TP/LPS-induced apoptosis and necroptosis. (A) and (B) Representative protein bands related to apoptosis and necroptosis after etanercept treatment, with tubulin as the loading control (n = 3–6). (C)–(J) Densitometric analyses of the bands in Fig. 2A and B. (K) Pictures of liver tissue sections analyzed by IHC for cleaved caspase-3 (top panels) and MLKL (bottom panels) (200 × ). Scale bar = 50 μm. Results were expressed as mean ± SEM and statistical analysis was performed using One-way ANOVA following by Tukey's multiple comparison test. *, #P < 0.05, **, ##P < 0.01, ***,###P < 0.001.