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. 2020 Feb 28;10(5):861–877. doi: 10.1016/j.apsb.2020.02.009

Figure 5.

Figure 5

Possible role of NF-κB and NF-κB-mediated pro-survival signals in TP/TNF-α-induced hepatic cell apoptosis. (A) L-02 cells were treated with TP (25 nmol/L) plus TNF-α (50 ng/mL) and were collected 30 min after TNF-α treatment for immunocytochemistry. Scale bar = 50 μm. (B)–(D) L-02 cells were treated with TP (25 nmol/L) plus TNF-α (50 ng/mL) and were collected 24 h after TNF-α application. Representative Western blots and relative intensity of protein bands of FLIPL and FLIPS with GAPDH as the loading control (n = 3–4). Results were expressed as mean ± SEM and statistical analysis was performed using One-way ANOVA following by Tukey's multiple comparison test. *, #P<0.05, **, ##P<0.01, ***, ###P<0.001; ns, no statistical difference.