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. 2020 Feb 28;10(5):861–877. doi: 10.1016/j.apsb.2020.02.009

Figure 6.

Figure 6

Protective role of FLIPS in TP/TNF-α-induced hepatic cell apoptosis. (A) and (B) Relative LDH release in L-02 cells transfected with siRNA or plasmid before the treatment of TP (25 nmol/L) and TNF-α (50 ng/mL) (n = 4). (C)–(F) Representative Western blots and relative intensity of protein bands of FLIPS and cleaved PARP in L-02 cells transfected with siRNA-NC or siRNA-FLIPS before the treatment of TP and TNF-α with GAPDH as the loading control (n = 4–5). (G)–(I) Representative Western blots and relative intensity of protein bands of FLIPS and cleaved PARP in L-02 cells transfected with pEX-1-NC or pEX-1-FLIPS before the treatment of TP and TNF-α with GAPDH as the loading control (n = 3). Results were expressed as mean ± SEM and statistical analysis was performed using One-way ANOVA following by Tukey's multiple comparison test. *P < 0.05, **P < 0.01, ***P < 0.001; ns, no statistical difference.