Figure 7.

Overexpression of FLIP_L protected mice from TP/LPS-induced hepatotoxicity. (A) Experimental procedure for the protective effects of FLIP_L on TP/LPS-induced hepatotoxicity. (B) and (C) Changes of serum ALT and AST in mice treated with TP and LPS after the transfection of AAV-NC and AAV-FLIP_L (n = 6). (D)–(H) Representative Western blots and relative intensity of the protein bands of RIPK1, P-MLKL, MLKL, and cleaved caspase-8 with GAPDH as the loading control (n = 4–6). (I) Pictures of liver sections analyzed by H&E staining (top panels), IHC staining for cleaved caspase-3 (middle panels) and MLKL (bottom panels) after the transfection of AAV-NC and AAV-FLIP_L (200 ×). Scale bar = 50 μm. Results were expressed as mean ± SEM and statistical analysis was performed using One-way ANOVA following by Tukey's multiple comparison test.  *, ^#P<0.05, **, ^##P<0.01, ***, ^###P<0.001; ns, no statistical difference.