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. 2020 Feb 14;100(6):887–899. doi: 10.1038/s41374-020-0406-7

Fig. 2. Nucleotide sequence analysis of mutations harbored by SGCD-KO pigs.

Fig. 2

a Cloned piglets generated from SGCD−/− cells. b PCR genotyping of the SGCD-KO cloned piglets. Lanes 1, 2, 4, and 5 show 2251 and 1546 bp bands corresponding to the mutated SGCD with 8 and 713 bp deletions in nuclear donor cell #49 (Clone #49). Lane 3 shows 2255 and 400 bp bands amplified from nuclear donor cell #26 (Clone #26) harboring 4 and 1859 bp deletions of SGCD. M: DNA marker. c DNA sequence analysis of SGCD in the cloned piglets. The SGCD mutations were identical in the cloned piglets and nuclear donor cells (arrows).