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. 2020 Feb 14;100(6):887–899. doi: 10.1038/s41374-020-0406-7

Fig. 3. Analysis of SGCD expression in the skeletal muscle of SGCD−/− pigs.

Fig. 3

a RT-PCR analysis of SGCD expression in WT and KO pigs. A 937 bp RT-PCR amplicon was detected in WT tissue, whereas 928 bp (*) and 748 bp (**) amplicons were detected in the KO tissues (M38-1, 2, 4, 5), corresponding to the sizes expected for the mutated alleles. b Western blot analysis of the skeletal muscle showed the presence of δ-SG in membrane-enriched skeletal muscle preparations of WT but not of KO pigs, regardless of age. α-Tubulin served as a loading control. Images of cropped gels/blots are shown. Original gels and western blots were run under the same experimental conditions.