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. 2020 Jun 8;10:9238. doi: 10.1038/s41598-020-65732-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Continuous exposure to 1.7 GHz LTE RF-EMF induced cell cycle delay at G1 phase in ASCs and Huh7 cells. (A–B) ASC and Huh7 cells were exposed to 1.7 GHz RF-EMF for 72 h at 1 SAR (A) or 2 SAR (B). The DNA content of the 1.7 GHz RF-EMF-exposed and the unexposed sham control cells was analyzed by flow cytometry (BD Bioscience) after PI staining. 100,000 cells were counted for each experiment. The FACS results were analyzed using Flowing software 2 and the distribution of cells in each stage of the cell cycle was calculated from the FACS results shown in the left panel using Flowing software 2. P < 0.05 (*), P < 0.01(**), P < 0.001(***), and P > 0.05 (non-significant, n.s.).