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. 2020 Jun 8;10:9209. doi: 10.1038/s41598-020-65836-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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M₁ mAChR activation within PRh is necessary for object memory updating in the PROMM task. (a) Intra-PRh microinjections (blue arrow) of the M₁ mAChR antagonist pirenzepine or its vehicle were administered immediately prior to object memory reactivation in the Y-apparatus (n = 10). (b) Object exploration in the vehicle/same alternate context condition was lower than object exploration in the pirenzepine/same alternate context condition, vehicle/different alternate context condition, and pirenzepine/different alternate context condition. **p < 0.01, ***p < 0.001.