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. 2020 Jun 8;10:9209. doi: 10.1038/s41598-020-65836-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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M₁ mAChR blockade within PRh does not disrupt contextual recognition memory. (a) Diagram of the procedure for the context recognition task. Rats study two identical contexts that are attached to arms of a Y-apparatus. After a 24-h delay, rats are presented one of the sample contexts and one novel context. Greater time spent exploring the novel context was considered indicative of intact memory for the sampled context. Intra-PRh microinjections (blue arrow) of pirenzepine or vehicle were administered immediately prior to the sample phase of the context recognition task (n = 18). (b) The choice discrimination ratio of the vehicle group and the pirenzepine group were both significantly greater than zero and did not differ from one another. *p < 0.05, **p < 0.01.