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. 2020 Jun 8;10:9209. doi: 10.1038/s41598-020-65836-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Schematic representation of cannula tip placements for microinjections targeting PRh from a representative cohort of cannulated rats. (a) Cannula implantation placements of cohort used for the pirenzepine experiment of this study (n = 10). Dotted lines above and below the marked cannula placements (black dots) indicate the dorsal and ventral borders of PRh. (b) Micrograph of a typical cannula guide tract extending to PRh.