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. 2020 Jun 8;11(6):437. doi: 10.1038/s41419-020-2635-5

Fig. 5. HIF-1α served as the target of circSLC25A16/miR-488-3p.

Fig. 5

a Bioinformatics prediction revealed the potential target effector protein for miR-488-3p. The wild type and mutant sequences for HIF-1αluciferase reporter vector were constructed. b Luciferase reporter assay demonstrated the activities of miR-488-3p mimics/control combined with the HIF-1α mRNA wild type/mutant. c RT-PCR revealed the HIF-1α mRNA expression in A549 cells transfected with miR-488-3p mimics or inhibitor. d Western blot and e RT-PCR revealed the HIF-1α protein and mRNA with circSLC25A16 overexpression (circSLC25A16 OE) transfection and miR-488-3p mimics co-transfection. **p < 0.01. *p < 0.05.