Fig. 4. In the absence of BIM, the ability of Dex to cause death of Bax^−/−Bak1^−/−, and Bmf^−/−Puma^−/− WEHI7 cells was reduced.

a Multiple independent clonal Bax^−/−Bak1^−/−, Bim^−/−Bmf^−/−Puma^−/− and Bax^−/−Bak1^−/−Bim^−/− WEHI7 lines were treated with 1 µM Dex and/or 10 µM QVD-OPh for 6 days. Cells were analyzed by flow cytometry for uptake of PI. Each symbol represents an independently derived clonal line. b Dot plots show independent clones from one of the three independent experiments shown in a. Numbers indicate the percent of PI-negative cells in a total of 10,000 cells analyzed per condition. c In two independent experiments, Bax^−/−Bak1^−/− parental, and three independent Bax^−/−Bak1^−/−Bim^−/− mutant p53^−/− T lymphoma cell lines were treated with 1 µM Dex and/or 10 µM QVD-OPh for 6 days. Cells were harvested, resuspended in PBS containing PI and analyzed by flow cytometry. d Dot plots show the parental Bax^−/−Bak1^−/− p53^−/− T lymphoma cell line and one of the three Bax^−/−Bak1^−/−Bim^−/− mutant p53^−/− T lymphoma cell lines shown in c. Numbers indicate the percentage of PI-negative cells from a total of 10,000 cells analyzed per condition.