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. 2020 Jun 8;11(6):442. doi: 10.1038/s41419-020-2599-5

Fig. 5. Dex-induced caspase activation and cytochrome c release in the absence of BAX and BAK1, but this failed to occur if BIM was also deleted.

Fig. 5

a Bax+/+Bak1+/+ (WT), Bax−/−Bak1−/−, Bax−/−Bak1−/−Casp9−/−, Bax−/−Bak1−/−Apaf1−/−, Bim−/−Bmf−/−Puma−/− and Bax−/−Bak1−/−Bim−/− clonal WEHI7 lines were treated with 1 µM Dex for indicated times. Cell lysates were analyzed by western blot using antibodies specific for cleaved Caspase-3, cleaved Caspase-9, and ACTIN. Note, the first 6 lanes of these blots are also shown in left panel of Fig. 2a. b Bax−/−Bak1−/− and Bax−/−Bak1−/−Bim−/− p53−/− T lymphoma lines were treated with 1 µM Dex for indicated times. Whole-cell lysates were analyzed by western blot using antibodies specific for cleaved Caspase-3, cleaved Caspase-9, and ACTIN. Note, the first 6 lanes of these blots are also shown in right panel of Fig. 2a. c Bax+/+Bak1+/+, Bax−/−Bak1−/−, Bax−/−Bak1−/−Casp9−/−, Bax−/−Bak1−/−Apaf1−/−, Bim−/−Bmf−/−Puma−/− and Bax−/−Bak1−/−Bim−/− WEHI7 lines were treated with 1 µM Dex for indicated times. Cytoplasmic extracted fractions were subjected to western blot analysis, using antibodies specific for cytochrome c (CYTC), and ACTIN. d Bax−/−Bak1−/− and Bax−/−Bak1−/−Bim−/− p53−/− T lymphoma lines were treated with 1 µM Dex for indicated times. Cytoplasmic fractions were subjected to western blot analysis, using antibodies specific for CYTC, and ACTIN.