Fig. 7. Deletion of BIM increased clonogenic survival of WEHI7 cells in response to Dex.

a One representative WEHI7-derived clone of each genotype (Bax^−/−Bak1^−/−, Bax^−/−Bak1^−/−Apaf1^−/− and Bax^−/−Bak1^−/−Bim^−/−) was cultured for 10 days in the presence or absence of 1 µM Dex. Cells were then washed, and plated in soft agar without Dex at a density of 5000 cells per well. Cells without Dex pre-treatment were plated at a lower density of 250 cells per well to generate countable numbers of colonies. After 14 days, the cloning efficiency was calculated by dividing the number of colonies by the number of cells initially plated in each well, and expressed as percentages. The results are from three independently conducted experiments. Photos of one of the three experiments are shown on the right. b Three independent WEHI7 cell clones from each genotype (Bax^−/−Bak1^−/−, Bim^−/−Bmf^−/−Puma^−/−Bax^−/−Bak1^−/−Apaf1^−/− and Bax^−/−Bak1^−/−Bim^−/−) were cultured for 10 days in the presence or absence of 1 µM Dex. Cells were then washed free of Dex, and plated in soft-agar medium for 14 days. Photos of one of the three experiments are shown on the right. c Four independent ihBIM Bax^−/−Bak1^−/−Bim^−/− WEHI7 cell clones were cultured for 10 days in the presence of 1 µM Dex and/or 1 µg/ml Dox. Cells were then washed free of Dex, and plated in soft-agar medium at a density of 4000 cells per well. Cells without Dex pre-treatment were plated at a lower density of 400 cells per well. Colonies were counted 14 days after plating.