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. 2020 Jun 8;10:181. doi: 10.1038/s41398-020-00864-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Western blot showing ApoE protein levels in CM and lysate of astrocytes treated with OGD-R and indicated lenti-virus. b CCK-8 assay showing the cell viability of endothelial cells treated with OGD-R and indicated lenti-virus (n = 4). c ROS production in endothelial cells treated with OGD-R and indicated lenti-virus (n = 4). d Caspase-Glo 3/7 assay showing caspase activity in endothelial cells treated with OGD-R and indicated lenti-virus (n = 4). e Apoptosis rate revealed by Annexin V/PI assay in endothelial cells treated with OGD-R and indicated lenti-virus (n = 4). ***P < 0.001.