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. 2020 Jun 8;10:9211. doi: 10.1038/s41598-020-66135-1

Figure 7.

Figure 7

DFAT cells differentiated to mural cells in Matrigel. (A) Schematic illustration of Matrigel plague assay. GFP-labelled DFAT cells embedded in Matrigel were subcutaneously injected into the cervical area of 10-week-old male C57BL/6 mice. (B) Immunofluorescence staining of the Matrigel with GFP, ASMA, and Hoechst at 14 days after the gel injection. The arrowheads indicate ASMA- and GFP-double positive vessels. Scale = 200 μm. (C) Immunofluorescence staining of the Matrigel with ASMA and vWF 14 days after the gel injection. Scale = 100 μm.