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. 2020 Jun 8;10:9174. doi: 10.1038/s41598-020-66116-4

Figure 2.

Figure 2

Juvenile-adolescent ELE results in improved novel object recognition memory in male and female mice. (a) OLM experimental design diagram. (b,c) Habituation analysis revealed male and female adolescent mice similarly habituated to OLM chambers, regardless of group, as demonstrated by significantly reduced distance traveled across trials. (d) OLM acquisition in 10-min trained male and female mice demonstrated no significant object discrimination. (e) During OLM testing, all mice demonstrated significant exploration of the novel-placed object, and there were no significant differences in DIs between groups. (f) No differences in total exploration during OLM testing in 10-min trained mice. (g) Mice trained for 3-min during OLM acquisition did not demonstrate object preference. (h) Juv ELE and juv-adol ELE male mice, and juv ELE female mice,  had significant preference for the object placed in a novel location when compared to sedentary controls, whereas adol ELE mice did not. (i) Total exploration times during OLM testing in 3-min trained mice did not differ across groups or sexes. Post hoc comparisons vs ‘no ELE’ group: (*p < 0.05; **p < 0.01; ***p < 0.005 vs no ELE) and within group comparisons vs OLM training DI: (#p < 0.05; ##p < 0.01; ###p < 0.005 vs training DI). N = 6–12 mice per group. Experimental design diagram generated with Biorender.com academic subscription.