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. 2020 Jun 8;11(6):425. doi: 10.1038/s41419-020-2641-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a HEK293 cells were transfected with a plasmid encoding Myc-PINK1 and/or hTERT-HA for 48 h. Total cell lysates were immunoprecipitated with anti-Myc antibody and immunoblotted with the indicated antibodies. Hsp90 served as a loading control. b Representative confocal images of endogenous PINK1 (green) and hTERT (red) immunostaining are shown. Scale bar = 5 μm. c Pearson′s correlation coefficient of the colocalization between PINK1 and hTERT in Fig. 1b was analyzed by Image J software. Data are presented as the mean ± SEM of three independent experiments (***p ≤ 0.001). d SH-SY5Y cell lysates were immunoprecipitated with anti-PINK1 IgG and immunoblotted with the indicated antibodies. The cell lysates were immunoprecipitated with pre-immune IgG as a negative control. e HEK293 cells were transfected for 48 h with a plasmid encoding Myc-PINK1 and/or hTERT-HA, and the resulting cell lysates were separated into cytosolic and membrane organelle fractions. The samples were then immunoprecipitated with anti-Myc antibody and immunoblotted with the indicated antibodies. Tubulin and VDAC served as markers for the cytosolic and the mitochondrial fractions, respectively.