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. 2020 Jun 8;11(6):425. doi: 10.1038/s41419-020-2641-7

Fig. 2. hTERT decreases PINK1 processing.

Fig. 2

a HEK293 cells were transfected for 48 h with a plasmid encoding PINK1-Myc alone or with increasing amounts of plasmid encoding hTERT-HA, and the resulting cell lysates were immunoblotted with the indicated antibodies. b HEK293 cells were transfected for 48 h with a plasmid encoding PINK1-Myc alone or with a plasmid encoding hTERT-HA. The cells were treated with 10 μM MG132 for the indicated times and the resulting cell lysates were immunoblotted with the indicated antibodies. c HEK293 cells were mock-transfected or transfected with a plasmid encoding hTERT-HA. The cells were treated with 10 μM MG132 for the indicated times and the resulting cell lysates were immunoblotted with the indicated antibodies. d HEK293 cells were transfected for 48 h with a plasmid encoding PINK1-Myc alone or with a plasmid encoding hTERT-HA. The cells were treated with 20 μg/ml cycloheximide for the indicated times and the resulting cell lysates were immunoblotted with the indicated antibodies. e HEK293 cells were mock-transfected or transfected with a plasmid encoding hTERT-HA. The cells were treated with 20 μg/ml cycloheximide for the indicated times and the resulting cell lysates were immunoblotted with the indicated antibodies. All graph data are presented as the mean ± SEM of three independent experiments (**p ≤ 0.01; a–e). Statistical analyses were performed using the IBM SPSS Statistics software (version 23.0).