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. 2020 Jun 8;11(6):435. doi: 10.1038/s41419-020-2645-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a, b RNA pull-down assay showing the endogenous proteins HDAC3 and WRD5 interact with biotinylated ANRIL in ox-LDL induced HASMCs, as stained by coomassie blue a and western blots b. c RNA pull-down assay to determinate the binding regions of ANRIL with HDAC3 and WRD5 using truncated ANRIL (Δ1: 1–500, Δ2: 501–1000, Δ3: 1001–1500, Δ4: 1501–2177). d–f Protein immunoprecipitation assay showing ANRIL binds with HDAC3 and WRD5, as pull-downed RNA detected by rapid staining of agarose gels d and qPCR e, f.