Fig. 4.

Adipose tissue inflammatory status. WT, wild-type mice; 4ko, Casp1/Casp11/NE/PR3 knockout mice. a Representative image of adipose tissue histology in WT mice and Casp1/Casp11/NE/PR3 knockout mice that received a HFD intervention. b gWAT weight reported to total body weight in the four groups of mice. c Crown-like structures (CLS) count in gWAT in both WT and Casp1/Casp11/NE/PR3 knockout mice that received a HFD. d Adiponectin concentration in plasma at the end of the diet intervention. All samples were measured in a single replicate. e Leptin concentration in plasma at the end of the diet intervention. All samples were measured in a single replicate. f mRNA fold induction of several genes in the gWAT in the four groups of mice. All samples were measured in duplicates. Data is represented as mean ± SEM. *p < 0.05, effect of the diet; **p < 0.01, effect of the diet; ***p < 0.001, effect of the diet; ###p < 0.001, effect of the genotype.