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. 2020 Mar 30;48(6):1821–1836. doi: 10.1007/s10439-020-02492-2

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Schematic diagrams of (a) the in vitro hybrid mock circulatory loop system, (b) the ex vivo isolated beating heart setup, (c) the 4D-flow MRI setup. Air tank 1 for positive pressure (AT1 Pos); air tank 2 for vacuum pressure (AT2 Vac); regulator valve (RegV); solenoid valve (SoV); left ventricular reservoir (LVR); aortic reservoir (AoR); preload reservoir (PR); venous reservoir (VR); oxygenator (Oxy); adjustable resistance clamp (AdjRes); left atrium (LA); left ventricle (LV); right atrium (RA); right ventricle (RV).