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. 2020 Jun 2;10:732. doi: 10.3389/fonc.2020.00732

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Copyright © 2020 Zhang, van Die, Tefsen, van Vliet, Laan, Zhang, ten Dijke, Wuhrer and Belo.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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O-glycosylation analysis in PaTu-S and PaTu-T by PGC nano-LC-ESI-MS/MS. (A) Combined extracted ion chromatograms (EICs) of O-glycans derived from PaTu-S and PaTu-T cell lines. (B–M) Relative abundance of structural O-glycan classes derived from 0.5 million PaTu-S and PaTu-T cells on PGC nano-LC-ESI-MS/MS in negative ion mode (displayed as mean relative abundance plus standard deviation; N = 3). (B) Normalized total O-glycan content (estimated), (C) Core2/4 O-glycan structure, (D) Sialylated Tn antigen, (E) T antigen, (F) Terminal GlcNAc, (G) Terminal GalNAc, (H) α2,3-sialylation of Gal, (I) α2,6-sialylation of Gal, (J) α2,6-sialylation of GalNAc, (K) α1,2-fucosylation, (L) α1,3/4-fucosylation, and (M) sLe^A.