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. 2020 Jun 2;11:984. doi: 10.3389/fimmu.2020.00984

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Copyright © 2020 Malherbe, Wibmer, Nonyane, Reed, Sather, Spencer, Schuman, Guo, Pandey, Robins, Park, Fuller, Sacha, Moore, Hessell and Haigwood.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Longitudinal heterologous neutralizing antibodies elicited by CAP257 vaccine strategies in rabbits. (A–H) Rabbit serum samples after the second (week 6), third (week 14), and fourth immunization (week 22) were tested for neutralization of a panel of recombinant heterologous viruses in the TZM-bl assay. (I) Summary by group of all viruses neutralized. A decrease in RLU from a serum dilution <50 was considered as non-specific cell death and no neutralization. Data are expressed as ID₅₀, serum dilution that neutralized 50% of the infecting virus. Box and whisker plots show mean ID₅₀ for each group at each immunization timepoint shown with IQR and were calculated in GraphPad Prism 8.0; ANOVA, Kruskil–Wallis test; P-values are shown.