Fig. 8. Blebbistatin decreases ATG9A trafficking and neuronal autophagy caused by cerebral ischemia/reperfusion.

Mice were treated with 1 h of cerebral ischemia and reperfusion for 2 h. a Confocal microscopy was used to detect NMMHC IIA (blue), F-actin (red), and Neun (green). Bar: 50 µm. b Co-IP was used to detect protein interactions between actin and NMMHC IIA upon MCAO/R. C ATG9A (green), TGN46 (red), and DAPI (blue) were detected by confocal microscopy. Bar: 20 µm. d, e Beclin and LC3B-II expression are depicted by immunoblotting. Results are were expressed as the mean ± SD, n = 3. ^##P < 0.01 and ^###P < 0.001 vs. sham group; ^*P < 0.05, ^**P < 0.01, and ^***P < 0.001 vs. model group. f, g Then confocal microscope was used to detect Beclin 1/LC3B (green) and Neun (red). Bar: 20 µm.