Skip to main content
. 2020 Jun 8;11:2866. doi: 10.1038/s41467-020-16499-9

Fig. 1. Structure of the CKB substrate bound to the ASB9 substrate receptor and adapter proteins ELOB/ELOC.

Fig. 1

a Cryo-EM density of CKB-ASB9-ELOB/C, colored by subunit, with a final overall resolution of 4.1 Å. b Molecular model for CKB-ASB9-ELOB/C complex. The model was built by fitting crystal structures (CKB: 3DRB; ASB9/ELOB/C: 3ZKJ), de novo building of small regions using RosettaCM, and overall refinement using Rosetta Relax. c The ASB9 N-terminus inserts as one helical turn into the active site of one CKB monomer positioning the ASB9 aspartate D32, which was shown to be critical for high affinity binding, in a basic pocket (R132, N286, R292, R341).