Table 3.
Genomic loci, primer sequences, their final concentrations in PCR reactions and the lengths of the obtained amplicons.
| Primers pool | Locus | Primers sequences 5′-3′ | Primers concentration (pM) | Product length (bp) |
|---|---|---|---|---|
| P1 | PC | F: GTCGGCTCAGGGTCGCTGCTTGG; R: GCTCTAGGCCCGCTTTTCCCCGC | 0.3 | 195 |
| DC | F: CTGGAGGCCCTGCCCTTGCGG; R: CGCCGCACTCGGCCCGCTCC | 0.1 | 95 | |
| ATOH1 | F: CTCGGTGCAGCTGGACGCTCTGC; R: CCCGTCGCTTCTGTGGGACCGAG | 0.03 | 163 | |
| C1Ql2 | F: GCATGACTTCCAGGGCGGCGGTG; R: CCTGCCGCATGATCTGCGACCCT | 0.04 | 105 | |
| CNIH3 | F: TGTCCCGGGGCAGGAGGCAGTTC; R: CGGGCCCTGCAGAGGGTGTCCTA | 0.03 | 110 | |
| SLC9A3 | F: TTAGCGCGGCCAGAGTCGCTCCC; R: CTGGGCCTGGGGGCTTCGTTGTG | 0.03 | 122 | |
| SNAP25 | F: TAAGAGTCGCCCCGTGCGGGTGT; R: AGCGAGGGGCGGGAGGAAGTGG | 0.04 | 148 | |
| P2 | PC | F: GTGACGGTGCCACTCACGTCGCC; R: TTCCACTTCGTGCACCGCTCGGC | 0.08 | 197 |
| DC | F: GCCTGGAGGCCCTGCCCTTGC; R: CGCCGCACTCGGCCCGCTCC | 0.24 | 97 | |
| ADCY8 | F: GCCGGCGTGGGAGAGGACCACTG; R: AACAGCGGAGGAACCGGCTGGCG | 0.1 | 114 | |
| DPYS | F: CATCGTTGACCACGCGACCCCCG; R: TCGGTGGGGACCTTGCAGGAGGG | 0.32 | 154 | |
| IRF4 | F: GCCTCGTGGCTGAAGGGCAGCTC; R: AGCTCACCGCGCTCATGCCGAAC | 0.1 | 145 | |
| KCNQ2 | F: CCGGCGGCTGCAGAGATGGGAC; R: AGCTGTCTGTCCTGCCCCCTCGG | 0.07 | 105 | |
| PRR5 | F: CCCTGTTCAGCCTCCGCATTCCCA; R: CCCCAAGGCCTCTGCTGTCCCCT | 0.04 | 167 | |
| P3 | PC | F: GTCGGCTCAGGGTCGCTGCTTGG; R: AGGCCCGCTTTTCCCCGCTTGAG | 0.019 | 190 |
| DC | F: CTCCGCCGCCTCAGTAGCCTCC; R: AGCGCAACTTACTTTCCGCCTGC | 0.03 | 84 | |
| GRIK1 | F: GCAGTGACGCGGCTCCCCCTTTT; R: CACCAACGCGGGTGTAGCGGGTC | 0.015 | 124 | |
| PTGIS | F: GGACTGCCGAAAGCAAGGCAGGG; R: TCTGCGTGGCCCGGGTGGAAGAA | 0.04 | 103 | |
| SKOR2 | F: CAGAGTCCGCGGGCGGCGTGGAG; R: CCCCCGCAGGTAGTGGCCAACG | 0.03 | 145 | |
| SYNDIG1 | F: CCGCTAGGGCCTCCCTGGTCTGG; R: GAGCCGCTCCTCTTCGCTTGCCG | 0.02 | 152 | |
| TERT | F: GCGGAGCTGGAAGGTGAAGGGGC; R: GGGAAGCGCGGCCCAGACCC | 0.027 | 168 |