Fig. 3. xStAx-VHLL impedes colorectal cancer cell proliferation and restrains tumor formation in nude mice.
a, b Equal amount of LoVo cells (a) and HCT116 cells (b) were treated with the vehicle DMSO or 50 μM peptides at for 6 days, and the cell number was counted each day. SA-V SAHPA1-VHLL, xS xStAx, xS-V xStAx-VHLL. c BALB/C nude mice were subcutaneously injected with 3 × 106 LoVo cells pretreated with DMSO or 50 μM peptides as indicated. After 3 weeks, the mice were sacrificed. Tumor weight was shown in the right. n = 10 mice per group. d Tumor samples from c were homogenized for immunoblotting. Relative β-catenin protein level was quantified. The relative band intensity was quantified with ImageJ and normalized to GAPDH levels. Data from three tumor samples are displayed as the mean ± SD by two-way ANOVA (a, b) or one-way ANOVA (c, d). *P < 0.05, **P < 0.01, ***P < 0.001.