Fig. 4. xStAx-VHLL reduces the number of intestinal tumors in APC^min/+ mice.

a Eleven weeks-old APC^min/+ mice (Day 0) were intraperitoneally injected with DMSO or peptides (30 mg/kg) for 7 times at every other day (n = 6, 7, 6, 6, 6 mice for each group, respectively). Red circles indicate tumors. Tumor number of the whole intestinal tract was analyzed in the lower panel. SA-V SAHPA1-VHLL, xS xStAx, xS-V xStAx-VHLL. b The tissue of the APC^min/+ mice small intestine was homogenized and total proteins were extracted for immunoblotting. Right panel is the quantitation. c The AXIN2-lacZ Wnt reporter mice were intraperitoneally injected with peptides for seven times at very other day. Then the mice were sacrificed for β-galactosidase staining in intestine. Relative β-galactosidase intensity was quantified in the lower panel. Scale bar = 100 μm. Data are displayed as the mean ± SD by one-way ANOVA. *P < 0.05, **P < 0.01, ***P < 0.001.