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. 2020 Jun 9;6:35. doi: 10.1038/s41421-020-0171-1

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Twelve CRC patient-derived organoids, numbered from C-01 to C-12, were treated with DMSO and 10 μM xStAx-VHLL for 4 days. The organoids survival rate of each group was calculated at day 4. b The C-08 organoids were treated with DMSO and 10 μM xStAx-VHLL for 4 days. The trypan blue staining at day 4 indicated the dead organoids. Scale bar = 100 μm. c The percentage of survived C-08 organoids was calculated each day after xStAx-VHLL treatment. d The C-08 organoids were treated with DMSO or 10 μM xStAx-VHLL for 48 h and then harvested for immunoblotting. Relative β-catenin protein levels were quantified. e The C-08 organoids were treated with DMSO and 10 μM xStAx-VHLL for 4 days. Scale bar = 100 μm. f The percentage of survived organoids in C-11 group was calculated each day after xStAx-VHLL treatment. g The C-11 organoids were treated with DMSO or 10 μM xStAx-VHLL for 48 h and then harvested for immunoblotting. Relative β-catenin protein levels were quantified. The relative band intensity was quantified with ImageJ and normalized to GAPDH levels. Data from three independent experiments are displayed as the mean ± SD by two-way ANOVA (a, c) and one-way ANOVA (d, g). *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.