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. 2020 Jun 2;11:925. doi: 10.3389/fimmu.2020.00925

Figure 3.

Figure 3

VCAM-1, ICAM-1, and P-selectin expression by EC after stimulation with selected adipokines and therapeutics. Results were compared to non-stimulated controls. (A) mRNA expression of VCAM-1 by primary EC after stimulation with adiponectin (n = 5), visfatin (n = 10), resistin (n = 10), TNF-α (n = 9), or therapeutics (n = 8 each). Adipokines and therapeutics did not have any effect on expression of VCAM-1, whereas TNF-α significantly upregulated expression of VCAM-1 [76.8-fold (rhombus), 95% CI = 11.8–499]. (B) mRNA expression of ICAM-1 by primary EC after stimulation with adiponectin (n = 6), visfatin (n = 9), resistin (n = 9), TNF-α (n = 8), glucocorticoids (n = 4 each), or methotrexate (n = 5 each). Adiponectin led to a significant increased expression of ICAM-1 [−2.5-fold (rhombus), 95% CI = 0.26–0.6]. Resistin visfatin and therapeutics did not have any effect on expression of ICAM-1, whereas TNF-α significantly upregulated expression of ICAM-1 [37.02-fold (rhombus), 95% CI = 15–91.4]. (C) mRNA expression of P-selectin by primary EC after stimulation with adiponectin (n = 5), visfatin (n = 8), resistin (n = 8), TNF-α (n = 7), glucocorticoids (each n = 3), or methotrexate (each n = 4). Adipokines and therapeutics did not have any effect on expression of P-selectin. Tumor necrosis factor α significantly downregulated expression of P-selectin [−6.3-fold (rhombus), 95% CI = 0.069–0.37].