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. 2020 Jun 2;11:925. doi: 10.3389/fimmu.2020.00925

Figure 4.

Figure 4

Evaluation of RASFs binding to EC. A cell-to-cell binding assay using primary EC was used to evaluate the effect of the adipokines adiponectin, visfatin, resistin, and TNF-α (n = 8 each), glucocorticoids (prednisolone, dexamethasone, n = 7 each), and methotrexate (n = 6 each dosage). Unstimulated RASFs served as control. The percentage of change in adherent RASFs compared to unstimulated RASFs was calculated. Data in figure are shown in percentages as dot plots with estimated marginal means. Stimulation with selected adipokines increased and prednisolone decreased adhesion to EC in most of the cell samples (adiponectin: 26%, visfatin 19%, resistin 17%, prednisolone: −15%, NS).