Fig. 5.

CXCL9 expression following stimulation with RSV peptide or whole virus: Four subjects (matched study visit 12 and 13; 2-weeks and 4-weeks post-RSV infection, respectively) PBMC were re-stimulated with RSV specific peptides or whole virus. (A) Representative bivariate plots (single subject; pre-gated on CD3⁺ T cells) showing expression of CXCL9 and CD4 as measured by flow cytometry (arcsinh transformed/scaled; 50,000 events each). Blue line indicates CD4⁺CXCL9 median fluorescence intensity (MFI) for the DMSO control; red line indicates MFI for the respectively labeled stimulation condition. Red dot overlays indicate meta-clustered cell events as assigned by FlowSOM (clustering algorithm). Frequency of the CXCL9⁺CD4⁺ cluster (B) and MFI of CXCL9⁺ on bulk CD4⁺ (C) are shown; black line indicates respective median value. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.) (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)