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Fathead minnow primer sequences targeting three tyrosinase (tyr) CRISPR RNA (crRNA) strands for polymerase chain reaction and mutation verification analysis. FW = forward primer, RV = reverse primer.
| Target strand | Amplicon size (bp) | Primer | Sequence (5′ → 3′) |
|---|---|---|---|
| tyr-crRNA-A | 301 | FW | GGTGTGCGGATCCCTTTC |
| RV | GGTGTTCTTCGCCAGATTCA | ||
| tyr-crRNA-B | 203 | FW | CCTGAATCTGGCGAAGAACA |
| RV | ATTCGTGCGCAAAGTCAATATC | ||
| tyr-crRNA-C | 261 | FW | CTGTAGTTTATGTGTACGGGTGT |
| RV | CGTGTTCCTGAAGCTCATGT | ||
