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. 2020 Apr 30;12(5):1122. doi: 10.3390/cancers12051122

Table 2.

List of oligonucleotides used in the study.

Target Strand Sequence Application Product Size (bp)
GAPDH For
Rev
GTCAGTGGTGGACCTGACCT
AGGGGAGATTCAGTGTGGTG
housekeeper, qPCR,
normalization
395
ß-actin For
Rev
TCGTGCGTGACATTAAGGAGAA
AGCAGCCGTGGCCATCT
housekeeper, qPCR,
normalization
71
TAPIR-1
(Ex3)
For
Rev
TTCATAGACCGTGCTTCCGT
TGTGAACCTGGAGCTTTCCT
qPCR TAPIR-1,
RNA expression
271
TAPIR-1
(Ex2-3)
For
Rev
GGGACAACTCGAAGCAAAGG
CTACAGAATGCGTGGTGTGG
qPCR TAPIR-1,
RNA expression
389
TAPIR-1
(Ex1-2)
For
Rev
TCTCAAAGCAGGCTCCATGA
CGTCTGCTTCGAGTCTTCCT
qPCR TAPIR-1,
RNA expression
249
TAPIR-2
(Ex1-2)
For
Rev
GACTTCACCACTCACCAGGA
AGGGACCCGTCTTATGTGAC
qPCR TAPIR-2,
RNA expression
237
AURKA For
Rev
TTGAACACCCCTGGATCACA
AGCGTTCTAGATTGAGGGCA
Validation of microarray,
qPCR, RNA expression
280
CCNB1 For
Rev
TGGTGCACTTTCCTCCTTCT
TTAGCATGCTTCGATGTGGC
Validation of microarray,
qPCR, RNA expression
420
CCNB2 For
Rev
GCTGAACTCAAAAGCCGTCA
ACTGGTCTGAGAAGAGGTTTCA
Validation of microarray,
qPCR, RNA expression
306
p21CIP1/
CDKN1A
For
Rev
TGTCTTGTACCCTTGTGCCT
AAGATGTAGAGCGGGCCTTT
Validation of microarray,
qPCR, RNA expression
480
E2F1 For
Rev
CTCTAACTGCACTTTCGGCC
CCTCCTCCCCTTTGCTGATT
Validation of microarray,
qPCR, RNA expression
360
HIST1H1B For
Rev
ACTCCGAAGAAGGCGAAGAA
GCTTTAGGTTTTGCTGCTTTGG
Validation of microarray,
qPCR, RNA expression
321
Survivin/
BIRC5
For
Rev
TGATGAGAGAATGGAGACAGAG
ACAGCAGTGGCAAAAGGAG
Validation of microarray,
qPCR, RNA expression
223
MYBL2 For
Rev
CAGCAATGCCAGTACAGGTG
CTCAGGGTTGAGGTGGTTGT
Validation of microarray
qPCR
198
CCNE1 For
Rev
GCAGGATCCAGATGAAGAAATG
GGGTCTGCACAGACTGCAT
Validation of microarray
qPCR
96
CDC25C For
Rev
CTCAGATGCTGGAGGAAGAATC
GGGACGATGGGCTTCTTCAG
Validation of microarray
qPCR
283
FOXM1 For
Rev
CGTGGATTGAGGACCACTTT
GGCTTAAACACCTGGTCCAA
Validation of microarray
qPCR
187
MKI67 For
Rev
CCAGCTTCCTGTTGTGTCAA
AGCCGTACAGGCTCATCAAT
Validation of microarray
qPCR
129
KIF23 For
Rev
GTAGAGTGGCAGCCAAACAGC
CTGATCAGGTTGAAAGAGTAAAGGC
Validation of microarray
qPCR
197
AR For
Rev
GCCAGCAGAAATGATTG
AGCCTCTCCTTCCTCCTG
qPCR AR,
RNA expression
150
BRCA1 For
Rev
CAGCTTGACACAGGTTTG
GGATTTTCGGGTTCACTC
qPCR,
RNA expression
155
ATR For
Rev
AACCTCCGTGATGTTGCTTG
AATGACAGGAGGGAGTTGCT
qPCR,
RNA expression
164
CHEK1 For
Rev
TCAGACTTTGGCTTGGCAAC
GGTTGGTCCCATGGCAATTC
qPCR,
RNA expression
194