Table 10.
The forward primer adaptor sequence and the six base pair tags/unique barcode sequences.
| Primer ID | Forward Primer 5’-3’ | Unique 6 Nucleotide Tag |
|---|---|---|
| PA-V4-1 | CCATCTCATCCCTGCGTGTCTCCGACTCAG | AGCAGC |
| PA-V4-2 | CCATCTCATCCCTGCGTGTCTCCGACTCAG | CTCAGC |
| PA-V4-3 | CCATCTCATCCCTGCGTGTCTCCGACTCAG | AGAGAG |
| PA-V4-4 | CCATCTCATCCCTGCGTGTCTCCGACTCAG | AGATGC |
| PA-V4-5 | CCATCTCATCCCTGCGTGTCTCCGACTCAG | AGCATG |
| PA-V4-6 | CCATCTCATCCCTGCGTGTCTCCGACTCAG | ATCATC |
| PA-V4-7 | CCATCTCATCCCTGCGTGTCTCCGACTCAG | ATCTGC |
| PA-V4-8 | CCATCTCATCCCTGCGTGTCTCCGACTCAG | ATGAGC |
| PA-V4-9 | CCATCTCATCCCTGCGTGTCTCCGACTCAG | ATGATG |
| PA-V4-10 | CCATCTCATCCCTGCGTGTCTCCGACTCAG | ATGCAG |
| PA-V4-11 | CCATCTCATCCCTGCGTGTCTCCGACTCAG | ATGCTC |
| PA-V4-12 | CCATCTCATCCCTGCGTGTCTCCGACTCAG | CAGAGC |
| PA-V4-13 | CCATCTCATCCCTGCGTGTCTCCGACTCAG | CAGATG |
| PA-V4-14 | CCATCTCATCCCTGCGTGTCTCCGACTCAG | CAGCAG |
| PA-V4-15 | CCATCTCATCCCTGCGTGTCTCCGACTCAG | CAGCTC |
| PA-V4-16 | CCATCTCATCCCTGCGTGTCTCCGACTCAG | CATCTG |
| PA-V4-17 | CCATCTCATCCCTGCGTGTCTCCGACTCAG | CATGAG |
| PA-V4-18 | CCATCTCATCCCTGCGTGTCTCCGACTCAG | CTCATG |
| PA-V4-19 | CCATCTCATCCCTGCGTGTCTCCGACTCAG | CTGATC |
| PA-V4-20 | CCATCTCATCCCTGCGTGTCTCCGACTCAG | CTGCTG |