Figure 1.

Immunoblotting analysis of human articular chondrocyte (HAC) lysates following transfection with miR-145 mimics. HACs were transfected with control (C) or miR-145 (145) mimics and immunoprecipitation was performed with an anti-Ago2 antibody (Ago2) or an IgG control, as indicated. I (Input): 2.5% of the total lysate submitted to immunoprecipitation (IP); FT (Flow-through): 5% of the extract recovered after IP. (A) Samples were separated by SDS-PAGE and proven with the anti-Ago2 antibody to determine the presence of Ago proteins. (B) MiR-145 bound to Ago2 following immunoprecipitation, in comparison with IgG, was estimated by RT-qPCR. MiR-145 overexpression resulted in ~40 fold increased in the amount of miR-145 bound to Ago2.