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. 2020 Apr 25;12(5):1070. doi: 10.3390/cancers12051070

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Construction of recombinant novel oncolytic viruses (vAng1, vTRAIL, NOV). (A) Schematic diagram of virus construction. Wyeth strain vaccinia virus was used for recombination. vSC20 is vaccinia growth factor (VGF) deleted vaccinia virus. vSC65 is virus thymiding kinase (TK) deleted vaccinia virus. vAng1 was constructed by deletion of VGF and insertion of human angiopoeitin 1 (Ang1); vTRAIL was constructed by deletion of virus TK and insertion of human TNF-related apoptosis-inducing ligand (TRAIL); and NOV was constructed by deleting both VGF and TK and inserting Ang1 and TRAIL, respectively, in their place. (B) Western blotting was used to detect TRAIL and Ang1 in U2OS cells 48 h after virus infection (0.1 MOI). Uncropped blots of Figure 1B are shown in Figure S1.