Figure 7.

Metabolite set extension. (A,B) Reactions are depicted by black arrows, metabolites are depicted by lettered boxes. Reactions associated with metabolites are used to identify metabolites one or more steps away from the primary reaction. The maximum distance is the first studied parameter during the development of the cross-omics method. (B) Extension stringency threshold: e.g., if metabolite D2 is present in more reactions than the defined threshold, metabolite E3 is not included in the gene-specific metabolite set via metabolite D2. The extension stringency threshold is the second studied parameter during the development of the cross-omics method.