Figure 6.

Luminal CD44^−/low/CD24^−/low cells cause metastasis in NSG mice. (A) Schematic representation of the xenotransplant experiment. Female NSG mice were injected with 5 × 10⁴ cells from CD44^high/CD24^−/low, CD44^−/low/CD24^−/low and siALDH1 populations. The mammary fat pad (MFP) and lungs were compared for tumorigenic and metastatic potential after 8 weeks of incubation. (B) Representative images of H&E staining of the site of injection in the MFP. (C) Representative images of H&E staining of lung metastasis. (D) Analysis of tumor area in the MFP of NSG mice. (E) Analysis of lung tumor foci. (F) Comparison of lung tumor metastatic foci between non-si and siALDH1A1 in HER2-OE cell lines. Data represent the mean ± SD of three independent experiments; * p < 0.05 and ** p < 0.01. Scale bar = 200 μm.