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. 2020 May 14;12(5):1239. doi: 10.3390/cancers12051239

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© 2020 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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High tumorigenicity in MDA-MB-231 is not CD44 dependent. Analysis of the CD44^high/CD24^−/low and CD44^−/low/CD24^−/low populations in MDA-MB-231. Cells from short-term (7-days) and long-term (21 days) mammosphere culture were isolated by FACS (A) and injected in the MFP of female NSG mice as described earlier. (B) Representative images of H&E-stained lung metastasis. (C) Representative images of solid tumors. Scale bar = 0.5 cm. Comparison of lung metastatic foci (D), tumor volume (E), and survival rate (F). Data represent the mean ± SD of three independent experiments. Scale bar = 200 μm.