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New all-in-one plasmids for controlling an mAID-fused protein. (A) Schematic illustration of pAID5.1-N/C and pAID5.2-N/C. A multi-cloning site (MCS) for cDNA cloning is shown in blue. (B) Reporter depletion in a clone derived from HCT116 cells. This clone was generated by introducing a plasmid based on pAID5.4-N. An mAID–EGFP–NLS reporter was detected after incubating the cells with or without 500 μM IAA for 4 h.
