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. 2020 May 14;9(5):376. doi: 10.3390/pathogens9050376

Figure 2.

Figure 2

Detailed analysis of ORF3–IKBKB region interaction. Schematic representation showing the full-length IKBKB and those with truncated constructs (upper panel). The plasmid expressing ORF3-Myc was co-transfected with plasmids expressing each of truncated IKBKB with Flag-tag at C-terminus in HEK293T cells. At 24 hpt, cleared lysates were subjected to immunoprecipitation using anti-Myc beads. The immunoprecipitated proteins were probed with rabbit anti-Flag and anti-Myc antibodies. KN, Kinase domain; ULD, ubiquitin-like domain; SDD, scaffold/dimerization domain; NBD, NF-κB essential modifier (NEMO) binding domain; ΔKN, deleted kinase domain.